the oncolytic effect of respiratory syncytial virus (rsv) in human skin cancer cell line, a431
نویسندگان
چکیده
conclusions this study delineated the beneficial role of rsv for growth regulation of skin cancer cells and highlighted the involvement of rsv in the induction of apoptosis in a431 cells. these findings might conduct evidence into the oncolytic properties of rsv in the skin cancer. further studies are required to indicate intracellular targets for rsv-induced apoptosis in skin cancer cells. results the results indicated that a431 cell growth was inhibited following infection by rsv in a dose- and time-dependent manner. the most growth inhibitory effect of rsv was occurred at the moi of 3, and 48 hour after infection. the inhibitory effect of rsv on the cell growth was accompanied by the induction of apoptosis in the skin cancer cells. the percentages of early and late apoptotic cells were increased following exposure to rsv in a concentration- and time-dependent manner. materials and methods plaque assay was used to determine rsv titers. the cytotoxic effect of rsv in a431 (skin carcinoma cell line) was determined using mtt assay. the detection of apoptosis was performed via annexin-v-fitc staining method and analyzed with flow cytometry. background oncolytic viruses have become of noticeable interest as a novel biological approach for selectively infecting cancer cells and triggering apoptosis in a number of malignant cells. many researches are devoted to characterize more viruses with oncolytic properties. objectives evidences on the oncolytic feature of respiratory syncytial virus (rsv) are conflicting; therefore, this study was designed to elucidate the possible role of rsv on the modulation of cell growth and apoptosis in the skin cancer cells.
منابع مشابه
The Oncolytic Effect of Respiratory Syncytial Virus (RSV) in Human Skin Cancer Cell Line, A431
BACKGROUND Oncolytic viruses have become of noticeable interest as a novel biological approach for selectively infecting cancer cells and triggering apoptosis in a number of malignant cells. Many researches are devoted to characterize more viruses with oncolytic properties. OBJECTIVES Evidences on the oncolytic feature of respiratory syncytial virus (RSV) are conflicting; therefore, this stud...
متن کاملIdentification of Respiratory Syncytial Virus (RSV) Genome in the Stool of a Child with Acute Gastroenteritis
Case Report: Some viruses have been reported to cause respiratory and gastroenteric infections simultaneously. In this case we present isolation of human respiratory virus (RSV) type B from diarrheal sample of a 12 months' child with acute gastroenteritis. Results: The results indicated the presence of RSV subtype B genome in all three stool samples. Moreover, no sign of co-infections with oth...
متن کاملWhat is respiratory syncytial virus (RSV)?
one of the most common causes of bronchiolitis and pneumonia in children under 1 year of age in the United States. It is a common reason for young infants to be hospitalized during the RSV season. RSV can also cause serious respiratory illness in older adults. Most healthy people recover from RSV infection in 1 to 2 weeks. However, infection can be severe in a few, usually among those who are a...
متن کاملoncolytic effect of newcastle disease virus af2240 strain on the mcf-7 breast cancer cell line
0
متن کاملEpidemiological Changes of Respiratory Syncytial Virus (RSV) Infections in Israel
RSV is the leading cause of lower respiratory-tract infections in infants and therefore demands in-depth epidemiological characterization. We investigated here the distribution of RSV types in Israel between the years 2005-2012. Clinical samples were collected from 11,018 patients hospitalized due to respiratory illnesses and were evaluated for the presence of various respiratory viruses, inclu...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian red crescent medical journalجلد ۱۵، شماره ۱، صفحات ۶۲-۷
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023